Edvotek® Exploring Human Origin by PCR Amplification of Mitochondrial DNA

In this experiment, students will isolate their mitochondrial DNA and use the Polymerase Chain Reaction (PCR) to amplify two separate regions of the mitochondrial genome. Results are analysed using agarose gel electrophoresis.

Group Size: For 25 students

Time Required:
Extraction - 50 minutes
PCR Setup - 10 minutes
PCR - 2 hours
Electrophoresis - 60 minutes
Staining - 5 minutes to overnight

Kit Includes: Instructions, PCR EdvoBeads™ PLUS, universal DNA buffer, TE buffer, mitochondrial LyphoPrimer™ mix, EdvoQuick™ DNA ladder, LyphoControl™, Proteinase K, UltraSpec-Agarose™, TBE electrophoresis buffer powder, SYBR® Safe stain, FlashBlue™ stain, microcentrifuge & PCR tubes, disposable plastic cups, salt packets, and a 15 mL conical tube.

All You Need:
DNA electrophoresis, thermal cycler, micropipettes: 5-50 µl, UV or blue light transilluminator, white light box (optional), (2) water baths, microcentrifuge & microwave or hot plate.

Storage: Some components require freezer storage upon receipt