Edvotek® Restriction Enzyme Analysis Of DNA Kit
£96.96
£80.80
An introduction to restriction enzyme digest, using bacteriophage λ DNA and Eco R1 enzyme. Separation on agarose gives 6 bands as enzymes are cut at 6 specific sites: In the kit the digest has already been done, and samples are ready to load on gel, and analyse.
Pack Size:
1
In stock
Chilled
Chilled
NEW & IMPROVED - Now for 10 Lab Groups!
Analysis of Eco RI Cleavage Patterns of Lambda DNA
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
• Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences
• Understand how electrophoresis separates differently-sized molecules
• Perform agarose gel electrophoresis
• Load, run, analyse, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA
Group Size: For 10 Gels
Time Required: Complete in 60 minutes
Kit Includes: Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 mL (or 0.5 mL) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis buffer (50X), practice gel loading solution, FlashBlue™ DNA stain.
All You Need: DNA electrophoresis, micropipettes: 5-50 µl (optional), white light box, & microwave or hot Plate.
Storage: Room temperature stable. Storage of ready-to-load QuickStrip™ samples in the refrigerator is recommended.
Analysis of Eco RI Cleavage Patterns of Lambda DNA
This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
• Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences
• Understand how electrophoresis separates differently-sized molecules
• Perform agarose gel electrophoresis
• Load, run, analyse, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA
Group Size: For 10 Gels
Time Required: Complete in 60 minutes
Kit Includes: Instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 mL (or 0.5 mL) microcentrifuge tubes), UltraSpec-Agarose™, Electrophoresis buffer (50X), practice gel loading solution, FlashBlue™ DNA stain.
All You Need: DNA electrophoresis, micropipettes: 5-50 µl (optional), white light box, & microwave or hot Plate.
Storage: Room temperature stable. Storage of ready-to-load QuickStrip™ samples in the refrigerator is recommended.
| Pack Size | 1 |
|---|---|
| Gross Weight | 0.188 |
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