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AQA RP7: The Pigments Present in Leaves
Abstract:
Use of chromatography to investigate the pigments isolated from leaves of different plants, eg leaves from shade-tolerant and shade-intolerant plants or leaves of different colours.
Hints & Tips:
- The solvent is propanone: petroleum ether (bp 100-120C) in a ratio of 1:9. Label as flammable and harmful to the environment.
- The chromatograms will fade quickly once the experiment has finished, so mark the spot position(s) in pencil as soon as possible.
- You can use thin-layer chromatography plates as an alternative to paper. Ensure you hold the TLC plates by the edges.
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