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- WJEC1 - Animal and Plant Cells
- WJEC2 - Factors Affecting Enzyme Action
- WJEC3 - The Energy Content of Foods
- WJEC4 - Photosynthesis
- WJEC5 - Factors Affecting Transpiration
- WJEC6 - Distribution and Abundance of a Species
- WJEC7 - Variation in Organisms
- WJEC8 - Factors Affecting Reaction Time
- WJEC9 - Test Artificial Urine Samples for Protein and Glucose
- WJEC10 - The Effect of Antibiotics on Bacterial Growth
- A Level
- AQA
- RP1 - Enzyme Controlled Reactions
- RP2 - Root Tip Squash
- RP3 - Water Potential of Plant Tissue
- RP4 - Permeability of Cell-Surface Membranes
- RP5 - Heart Dissection
- RP6 - Aseptic Techniques
- RP7 - The Pigments Present in Leaves
- RP8 - Rate of Dehydrogenase Activity
- RP9 - Rate of Respiration
- RP10 - Animal Responses
- RP11 - Dilution Series of a Glucose Solution
- RP12 - Distribution of Species
- Edexel A
- CP1 - Effect of caffeine on heart rate in daphnia
- CP2 - Investigate the vitamin C content of food and drink
- CP3 - Investigate membrane structure
- CP4 - Effects of enzyme and substrate concentrations
- CP5 - Root tip squash to observe stages of mitosis
- CP6 - Looking at plant stems through a light microscope
- CP7 - Investigate plant mineral deficiencies
- CP8 - Determine the tensile strength of plant fibres
- CP9 - Investigate the antimicrobial properties of plants
- CP10 - A study on the ecology of a habitat
- CP11 - Investigate photosynthesis using isolated chloroplasts
- CP12 - Effect of temperature on an enzyme catalysed reaction
- CP13 - Effects of temperature on the development of organisms
- CP14 - Use gel electrophoresis to separate DNA fragments
- CP15 - Effect of different antibiotics on bacteria
- CP16 - Rate of respiration
- CP17 - Effects of exercise using spirometer data
- CP18 - Investigate habituation to a stimulus
- Edexel B
- CP1 - Factor affecting rate of an enzyme controlled reaction
- CP2 - Use a light microscope to draw cells
- CP3 - Root tip squash to show mitosis
- CP4 - Effect of sucrose conc. on pollen tube growth
- CP5 - Effect of temperature on beetroot membrane permeability
- CP6 - Determine water potential of a plant tissue
- CP7 - Dissect an insect to show structure of gas exchange system
- CP8 - Factors affecting water uptake using a potometer
- CP9 - Factors affecting rate of respiration
- CP10 - Effects of wavelength of light on photosynthesis
- CP11 - Presence of chloroplast pigments using chromatography
- CP12 - Rate of growth of bacteria in liquid culture
- CP13 - Isolate bacteria using streak plating
- CP14 - Effect of gibberellin on production of amylase
- CP15 - Sampling methods; estimating a population size
- CP16A - Effect of antibiotic factor on distribution of species
- CP16B - Effect of antibiotic factor on morphology of species
- OCR
- 1.1 - Microscopy - Using a Light Microscope to Study Mitosis
- 1.2 - Microscopy - Examination & Drawing of Blood Cells
- 1.3 - Microscopy - Using a Light Microscope to Examine Lung Tissue
- 2.1 - Dissection - Mammalian Heart
- 2.2 - Dissection - Stem
- 2.3 - Dissection - Muscle Fibres from a Chicken Wing
- 3.1 - Sampling techniques - Species Diversity
- 3.2 - Sampling techniques - Distribution of Plants
- 3.3 - Sampling Techniques - Species and Biotic/Abiotic Factor
- 4.1 - Rate of Reactions - Effect of Substrate Conc.
- 4.2 - Rate of Reactions - Effect of Enzyme Conc.
- 4.3 - Rate of Reactions - Effect of Temperature
- 5.1 - Colorimeter - Effect of Temperature on Membrane Permeability
- 5.2 - Colorimeter - Determining Glucose Concs.
- 5.3 - Potometer - Rate of Transportation
- 6.1 - Chromatography - Identify Amino Acids
- 6.2 - Electrophoresis - DNA Fragments
- 6.3 - Chromatography - TLC to separate photosynthesis pigments
- 7.1 - Microbiology - Effect of Antibiotics on Bacterial Growth
- 7.2 - Microbiology - Dilution Plating
- 7.3 - Microbiology - Transformation of Bacteria
- 8.1 - Cell Transport - Water Potential of a Potato
- 8.2 - Cell Transport - Osmosis in an Artificial Cell
- 8.3 - Cell Transport - Rate of Diffusion Through a Membrane
- 9.1 - Qualitative Testing - Proteins
- 9.2 - Qualitative Testing - Lipids
- 9.3 - Qualitative Testing - Glucose
- 10.1 - ICT - DNA Structure Using RasMol
- 10.2 - ICT - Turbidity Change in Microbial Growth in Sugars
- 10.3 - ICT - pH Change During Yoghurt Production
- 11.1 - Responses - Effect of Exercise on Pulse Rate
- 11.2 - Responses - Daphnia Heart Rate Response
- 11.3 - Responses - Phototropism
- 11.4 - Responses - Effect of Plant Hormones on Growth
- 12.1 - Research Skills - Respiration Rate of Saccharomyces
- 12.2 - Research Skills - Genetic Crosses in Fruit Flies
- 12.3 - Research Skills - Rate of Oxygen Production in Pondweed
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Edexcel B CP12: Rate of Growth of Bacteria in Liquid Culture
Abstract:
Microbial techniques: growth of bacteria in liquid culture.
Hints & Tips:
- All glass equipment needs to be sterilised before and after use. Cultures should be sterilised at the end of the experiment for destruction.
- Use the orange/red filter on the calorimeter (600nm wavelength).
- 1% Virkon® can be used (leave for 10 min before use).
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